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ecl chemiluminescent substrate  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology ecl chemiluminescent substrate
    Ecl Chemiluminescent Substrate, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+kit/Excellent+Chemiluminescent+Substrate+(ECL)Detection+Kit/pmc13682527-52-37-45
    Average 96 stars, based on 125 article reviews
    ecl chemiluminescent substrate - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: MicroRNA-155 acts as a potential prognostic and diagnostic factor in patients with ankylosing spondylitis by modulating SOCS3.
    Article Snippet: Background Ankylosing spondylitis (AS) is a progressive inflammatory disease.. Our primary objective was to explore the role of miR-155 and its targeted factors in AS pathogenesis.. Methods and results PBMCs were isolated from 30 AS patients and 30 healthy individuals using the Ficoll-hypaque isolation approach.

    Article Title: Whey Improves In Vitro Endothelial Mitochondrial Function and Metabolic Redox Status in Diabetic State
    Article Snippet: Then, the membranes were immunodetected with primary antibodies against SIRT3 (1:2000, PA5-86035, Invitrogen, Waltham, MA, USA), BAX (1:500, orb216030, Biorbyt, Cambridge, UK), Bcl-2 (1:500, E-AB-15522, Elabscience Biotechnology Inc., Houston, TX, USA), COX-IV (1:2000, MA5-15078, Invitrogen, Waltham, MA, USA), caspase-3 (1:1000, 9662, Cell Signaling Technology, Danvers, MA, USA), caspase-9 (1:1000, 9508, Cell Signaling Technology, Danvers, MA, USA), PARP (1:1000, ab194586, Abcam, Cambridge, UK), cyclin D1 (1:1000, ab134175, Abcam, Cambridge, UK), cyclin E1 (1:1000, ab33911, Abcam, Cambridge, UK), SREBP1 (1:1000, Abcam, ab28481, Cambridge, UK), PPAR-α (1:1000, Elabscience Biotechnology Inc., Houston, TX, USA, E-AB-32646), PPAR-γ (1:1000, Biorbyt, orb69095, Cambridge, UK), LDHA (1:1000, ThermoFisher Scientific, PA5-27406, Waltham, MA, USA), α-tubulin (1:5000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA), actin (1:3000, ab179467, Abcam, Cambridge, UK), and GAPDH (1:2000, ab9485, Abcam, Cambridge, UK). .. After 1 h incubation with HRP-labeled secondary antibody at room temperature, the ECL kit (E-IR-R301, Elabscience Biotechnology Inc., Houston, TX, USA) was applied on dried membranes and chemiluminescence detected by ChemiDoc Imaging System with Image Lab 6.0.1 software (Bio-Rad Laboratories, Milan, Italy). .. The density of protein bands was analyzed by ImageJ 1.52n version software (Wayne Rasband, National Institutes of Health, Bethesda, MD, USA) and then normalized to the relative loading control.

    Incubation:

    Article Title: Whey Improves In Vitro Endothelial Mitochondrial Function and Metabolic Redox Status in Diabetic State
    Article Snippet: Then, the membranes were immunodetected with primary antibodies against SIRT3 (1:2000, PA5-86035, Invitrogen, Waltham, MA, USA), BAX (1:500, orb216030, Biorbyt, Cambridge, UK), Bcl-2 (1:500, E-AB-15522, Elabscience Biotechnology Inc., Houston, TX, USA), COX-IV (1:2000, MA5-15078, Invitrogen, Waltham, MA, USA), caspase-3 (1:1000, 9662, Cell Signaling Technology, Danvers, MA, USA), caspase-9 (1:1000, 9508, Cell Signaling Technology, Danvers, MA, USA), PARP (1:1000, ab194586, Abcam, Cambridge, UK), cyclin D1 (1:1000, ab134175, Abcam, Cambridge, UK), cyclin E1 (1:1000, ab33911, Abcam, Cambridge, UK), SREBP1 (1:1000, Abcam, ab28481, Cambridge, UK), PPAR-α (1:1000, Elabscience Biotechnology Inc., Houston, TX, USA, E-AB-32646), PPAR-γ (1:1000, Biorbyt, orb69095, Cambridge, UK), LDHA (1:1000, ThermoFisher Scientific, PA5-27406, Waltham, MA, USA), α-tubulin (1:5000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA), actin (1:3000, ab179467, Abcam, Cambridge, UK), and GAPDH (1:2000, ab9485, Abcam, Cambridge, UK). .. After 1 h incubation with HRP-labeled secondary antibody at room temperature, the ECL kit (E-IR-R301, Elabscience Biotechnology Inc., Houston, TX, USA) was applied on dried membranes and chemiluminescence detected by ChemiDoc Imaging System with Image Lab 6.0.1 software (Bio-Rad Laboratories, Milan, Italy). .. The density of protein bands was analyzed by ImageJ 1.52n version software (Wayne Rasband, National Institutes of Health, Bethesda, MD, USA) and then normalized to the relative loading control.

    Article Title: The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study
    Article Snippet: The antibodies used were IL-6 (AffinityBiosciences), TMEM100 (Origene) and ß-actin (Elabscience). .. After overnight incubation, the PVDF membrane was incubated with horseradish peroxidase (HRP) labeled secondary antibody (Elabscience) for 1 hour, and then the luminescence was visualized by ECL kit (Elabscience). ..

    Article Title: A Dual-Responsive Fe₃O₄@ZIF-8 Nanoplatform Combining Magnetic Targeting and pH Sensitivity for Low Back Pain Therapy.
    Article Snippet: Low back pain (LBP) resulting from sciatic nerve compression presents major challenges in pain management, as traditional therapies provide only short-term relief and pose risks of systemic toxicity.. In this study, an innovative Fe3O4@ZIF-8-RVC (FZR) dual-responsive nanoplatform is introduced that integrates magnetic targeting with pH-sensitive, sustained drug release to overcome these limitations.. The FZR nanoplatform encapsulates ropivacaine (RVC) within the ZIF-8-coated Fe3O4 core, enabling precise and prolonged analgesia at the injury site through magnetic guidance and acid-triggered release.

    Software:

    Article Title: Whey Improves In Vitro Endothelial Mitochondrial Function and Metabolic Redox Status in Diabetic State
    Article Snippet: Then, the membranes were immunodetected with primary antibodies against SIRT3 (1:2000, PA5-86035, Invitrogen, Waltham, MA, USA), BAX (1:500, orb216030, Biorbyt, Cambridge, UK), Bcl-2 (1:500, E-AB-15522, Elabscience Biotechnology Inc., Houston, TX, USA), COX-IV (1:2000, MA5-15078, Invitrogen, Waltham, MA, USA), caspase-3 (1:1000, 9662, Cell Signaling Technology, Danvers, MA, USA), caspase-9 (1:1000, 9508, Cell Signaling Technology, Danvers, MA, USA), PARP (1:1000, ab194586, Abcam, Cambridge, UK), cyclin D1 (1:1000, ab134175, Abcam, Cambridge, UK), cyclin E1 (1:1000, ab33911, Abcam, Cambridge, UK), SREBP1 (1:1000, Abcam, ab28481, Cambridge, UK), PPAR-α (1:1000, Elabscience Biotechnology Inc., Houston, TX, USA, E-AB-32646), PPAR-γ (1:1000, Biorbyt, orb69095, Cambridge, UK), LDHA (1:1000, ThermoFisher Scientific, PA5-27406, Waltham, MA, USA), α-tubulin (1:5000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA), actin (1:3000, ab179467, Abcam, Cambridge, UK), and GAPDH (1:2000, ab9485, Abcam, Cambridge, UK). .. After 1 h incubation with HRP-labeled secondary antibody at room temperature, the ECL kit (E-IR-R301, Elabscience Biotechnology Inc., Houston, TX, USA) was applied on dried membranes and chemiluminescence detected by ChemiDoc Imaging System with Image Lab 6.0.1 software (Bio-Rad Laboratories, Milan, Italy). .. The density of protein bands was analyzed by ImageJ 1.52n version software (Wayne Rasband, National Institutes of Health, Bethesda, MD, USA) and then normalized to the relative loading control.

    Membrane:

    Article Title: D-alanine suppressed osteoclastogenesis derived from bone marrow macrophages and downregulated ERK/p38 signalling pathways.
    Article Snippet: Objectives: D-alanine is a residue of the backbone structure of Type I Lipoteichoic acid (LTA), which is a virulence factor in inflammation caused by gram-positive bacteria.. However, the role of D-alanine in infectious bone destruction has not been investigated.. We aimed to explore the role of D-alanine in the proliferation, apoptosis, and differentiation of osteoclasts.

    Article Title: The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study
    Article Snippet: The antibodies used were IL-6 (AffinityBiosciences), TMEM100 (Origene) and ß-actin (Elabscience). .. After overnight incubation, the PVDF membrane was incubated with horseradish peroxidase (HRP) labeled secondary antibody (Elabscience) for 1 hour, and then the luminescence was visualized by ECL kit (Elabscience). ..

    Article Title: A Dual-Responsive Fe₃O₄@ZIF-8 Nanoplatform Combining Magnetic Targeting and pH Sensitivity for Low Back Pain Therapy.
    Article Snippet: Low back pain (LBP) resulting from sciatic nerve compression presents major challenges in pain management, as traditional therapies provide only short-term relief and pose risks of systemic toxicity.. In this study, an innovative Fe3O4@ZIF-8-RVC (FZR) dual-responsive nanoplatform is introduced that integrates magnetic targeting with pH-sensitive, sustained drug release to overcome these limitations.. The FZR nanoplatform encapsulates ropivacaine (RVC) within the ZIF-8-coated Fe3O4 core, enabling precise and prolonged analgesia at the injury site through magnetic guidance and acid-triggered release.

    Labeling:

    Article Title: The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study
    Article Snippet: The antibodies used were IL-6 (AffinityBiosciences), TMEM100 (Origene) and ß-actin (Elabscience). .. After overnight incubation, the PVDF membrane was incubated with horseradish peroxidase (HRP) labeled secondary antibody (Elabscience) for 1 hour, and then the luminescence was visualized by ECL kit (Elabscience). ..



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